Coding

Part:BBa_K1218009:Design

Designed by: Trevor Kalkus, Gordon Wade, Alex Constantino, Alissa Greenberg   Group: iGEM13_Stanford-Brown   (2013-08-28)


CRISPR casBCDE E. coli (Modern)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1593
    Illegal BglII site found at 2778
    Illegal BamHI site found at 1723
    Illegal BamHI site found at 2084
    Illegal BamHI site found at 2981
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3702
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 263


Design Notes

None


Source

E. coli DH5alpha genome

References